# Most-cited Genetics and Biochemistry papers of 2020, with free PDFs

The 25 most-cited Genetics and Biochemistry research papers published in 2020, ranked by citations in OpenAlex, with their original abstracts and free PDFs where available.

1. [IQ-TREE 2: New Models and Efficient Methods for Phylogenetic Inference in the Genomic Era](https://nerdi.pages.dev/paper/W3005235420) · 16,861 citations · Free PDF
2. [The biology , function , and biomedical applications of exosomes](https://nerdi.pages.dev/paper/W3005104290) · 10,681 citations
3. [The mutational constraint spectrum quantified from variation in 141,456 humans](https://nerdi.pages.dev/paper/W3029661147) · 9,490 citations · Free PDF
4. [The STRING database in 2021: customizable protein–protein networks, and functional characterization of user-uploaded gene/measurement sets](https://nerdi.pages.dev/paper/W3107527779) · 8,105 citations
5. [Pfam: The protein families database in 2021](https://nerdi.pages.dev/paper/W3095583226) · 7,377 citations
6. [U1 snRNP regulates cancer cell migration and invasion in vitro](https://nerdi.pages.dev/paper/W2998840182) · 7,152 citations · Free PDF
7. [UniProt: the universal protein knowledgebase in 2021](https://nerdi.pages.dev/paper/W3112376646) · 6,435 citations
8. [The GTEx Consortium atlas of genetic regulatory effects across human tissues](https://nerdi.pages.dev/paper/W3133859624) · 5,524 citations · Free PDF
9. [KEGG: integrating viruses and cellular organisms](https://nerdi.pages.dev/paper/W3096828292) · 4,060 citations
10. [GetOrganelle: a fast and versatile toolkit for accurate de novo assembly of organelle genomes](https://nerdi.pages.dev/paper/W3084201551) · 3,998 citations · Free PDF
11. [Interaction between microbiota and immunity in health and disease](https://nerdi.pages.dev/paper/W3027658010) · 3,925 citations · Free PDF
12. [Merqury: reference-free quality, completeness, and phasing assessment for genome assemblies](https://nerdi.pages.dev/paper/W3085758516) · 3,657 citations · Free PDF
13. [The Gene Ontology resource: enriching a GOld mine](https://nerdi.pages.dev/paper/W3112600195) · 3,578 citations · Free PDF
14. [Using SPAdes De Novo Assembler](https://nerdi.pages.dev/paper/W3036135788) · 3,554 citations
15. [The repertoire of mutational signatures in human cancer](https://nerdi.pages.dev/paper/W3004480399) · 3,516 citations · Free PDF
16. [ResFinder 4.0 for predictions of phenotypes from genotypes](https://nerdi.pages.dev/paper/W3048398081) · 3,375 citations
17. [Pembrolizumab for Early Triple-Negative Breast Cancer](https://nerdi.pages.dev/paper/W3006930853) · 3,232 citations
18. [Pan-cancer analysis of whole genomes](https://nerdi.pages.dev/paper/W3006500278) · 3,102 citations · Free PDF
19. [The Role of Short-Chain Fatty Acids From Gut Microbiota in Gut-Brain Communication](https://nerdi.pages.dev/paper/W3003912482) · 3,009 citations
20. [Mitochondrial TCA cycle metabolites control physiology and disease](https://nerdi.pages.dev/paper/W2997046468) · 2,805 citations · Free PDF
21. [GenomeScope 2.0 and Smudgeplot for reference-free profiling of polyploid genomes](https://nerdi.pages.dev/paper/W3012094285) · 2,795 citations · Free PDF
22. [Identifying and removing haplotypic duplication in primary genome assemblies](https://nerdi.pages.dev/paper/W3002362821) · 2,767 citations · Free PDF
23. [Metabolic reprogramming and cancer progression](https://nerdi.pages.dev/paper/W3015450697) · 2,701 citations
24. [BlobToolKit – Interactive Quality Assessment of Genome Assemblies](https://nerdi.pages.dev/paper/W3007075333) · 2,653 citations · Free PDF
25. [NCBI Taxonomy: a comprehensive update on curation, resources and tools](https://nerdi.pages.dev/paper/W3048090444) · 2,628 citations

Canonical page: https://nerdi.pages.dev/papers/genetics-and-biochemistry/most-cited-2020