# Most-cited Genetics and Biochemistry papers of 2019, with free PDFs

The 25 most-cited Genetics and Biochemistry research papers published in 2019, ranked by citations in OpenAlex, with their original abstracts and free PDFs where available.

1. [Metascape provides a biologist-oriented resource for the analysis of systems-level datasets](https://nerdi.pages.dev/paper/W2928665623) · 14,653 citations · Free PDF
2. [OrthoFinder: phylogenetic orthology inference for comparative genomics](https://nerdi.pages.dev/paper/W2988396470) · 7,690 citations · Free PDF
3. [BioBERT: a pre-trained biomedical language representation model for biomedical text mining](https://nerdi.pages.dev/paper/W2911489562) · 7,279 citations · Free PDF
4. [Improved metagenomic analysis with Kraken 2](https://nerdi.pages.dev/paper/W2990618091) · 7,155 citations · Free PDF
5. [Interactive Tree Of Life (iTOL) v4: recent updates and new developments](https://nerdi.pages.dev/paper/W2927961455) · 5,988 citations
6. [Toward understanding the origin and evolution of cellular organisms](https://nerdi.pages.dev/paper/W2969718750) · 5,503 citations
7. [GTDB-Tk: a toolkit to classify genomes with the Genome Taxonomy Database](https://nerdi.pages.dev/paper/W2986925300) · 5,330 citations
8. [ShinyGO: a graphical gene-set enrichment tool for animals and plants](https://nerdi.pages.dev/paper/W2997022986) · 5,260 citations · Free PDF
9. [The Microbiota-Gut-Brain Axis](https://nerdi.pages.dev/paper/W2970686316) · 5,166 citations
10. [GEPIA2: an enhanced web server for large-scale expression profiling and interactive analysis](https://nerdi.pages.dev/paper/W2946657092) · 5,105 citations
11. [g:Profiler: a web server for functional enrichment analysis and conversions of gene lists (2019 update)](https://nerdi.pages.dev/paper/W2944545583) · 5,070 citations
12. [Breast Cancer Treatment](https://nerdi.pages.dev/paper/W2912993657) · 4,818 citations
13. [BEAST 2.5: An advanced software platform for Bayesian evolutionary analysis](https://nerdi.pages.dev/paper/W2901954177) · 4,585 citations · Free PDF
14. [Normalization and variance stabilization of single-cell RNA-seq data using regularized negative binomial regression](https://nerdi.pages.dev/paper/W2997280954) · 4,523 citations · Free PDF
15. [RAxML-NG: a fast, scalable and user-friendly tool for maximum likelihood phylogenetic inference](https://nerdi.pages.dev/paper/W2949867654) · 4,330 citations · Free PDF
16. [In silico analysis enabling informed design for genome editing in medicinal cannabis; gene families and variant characterisation](https://nerdi.pages.dev/paper/W2941307081) · 4,224 citations
17. [PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies](https://nerdi.pages.dev/paper/W2979811825) · 4,081 citations · Free PDF
18. [MetaBAT 2: an adaptive binning algorithm for robust and efficient genome reconstruction from metagenome assemblies](https://nerdi.pages.dev/paper/W2938868597) · 3,797 citations · Free PDF
19. [Short Chain Fatty Acids (SCFAs)-Mediated Gut Epithelial and Immune Regulation and Its Relevance for Inflammatory Bowel Diseases](https://nerdi.pages.dev/paper/W2921966151) · 3,795 citations · Free PDF
20. [The NLRP3 Inflammasome: An Overview of Mechanisms of Activation and Regulation](https://nerdi.pages.dev/paper/W2954782052) · 3,518 citations · Free PDF
21. [What is the Healthy Gut Microbiota Composition? A Changing Ecosystem across Age, Environment, Diet, and Diseases](https://nerdi.pages.dev/paper/W2909668868) · 3,440 citations · Free PDF
22. [TYGS is an automated high-throughput platform for state-of-the-art genome-based taxonomy](https://nerdi.pages.dev/paper/W2946204549) · 3,290 citations · Free PDF
23. [miRDB: an online database for prediction of functional microRNA targets](https://nerdi.pages.dev/paper/W2970358232) · 3,287 citations
24. [Overview of Extracellular Vesicles, Their Origin, Composition, Purpose, and Methods for Exosome Isolation and Analysis](https://nerdi.pages.dev/paper/W2962176767) · 3,248 citations · Free PDF
25. [CARD 2020: antibiotic resistome surveillance with the comprehensive antibiotic resistance database](https://nerdi.pages.dev/paper/W2982531601) · 3,231 citations

Canonical page: https://nerdi.pages.dev/papers/genetics-and-biochemistry/most-cited-2019