# Most-cited Genetics and Biochemistry papers of 2016, with free PDFs

The 25 most-cited Genetics and Biochemistry research papers published in 2016, ranked by citations in OpenAlex, with their original abstracts and free PDFs where available.

1. [MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets](https://nerdi.pages.dev/paper/W2311203695) · 44,250 citations
2. [Enrichr: a comprehensive gene set enrichment analysis web server 2016 update](https://nerdi.pages.dev/paper/W2345356016) · 10,867 citations
3. [VSEARCH: a versatile open source tool for metagenomics](https://nerdi.pages.dev/paper/W2513506562) · 10,810 citations
4. [MultiQC: summarize analysis results for multiple tools and samples in a single report](https://nerdi.pages.dev/paper/W2432815617) · 10,196 citations
5. [Complex heatmaps reveal patterns and correlations in multidimensional genomic data](https://nerdi.pages.dev/paper/W2406250479) · 10,174 citations
6. [Analysis of protein-coding genetic variation in 60,706 humans](https://nerdi.pages.dev/paper/W2256016639) · 9,662 citations · Free PDF
7. [Consistent Estimation in Mendelian Randomization with Some Invalid Instruments Using a Weighted Median Estimator](https://nerdi.pages.dev/paper/W2324862792) · 8,991 citations · Free PDF
8. [KEGG: new perspectives on genomes, pathways, diseases and drugs](https://nerdi.pages.dev/paper/W2559588208) · 8,900 citations
9. [deepTools2: a next generation web server for deep-sequencing data analysis](https://nerdi.pages.dev/paper/W2341539131) · 8,214 citations · Free PDF
10. [The Ensembl Variant Effect Predictor](https://nerdi.pages.dev/paper/W2417483443) · 8,173 citations · Free PDF
11. [Introducing EzBioCloud: a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies](https://nerdi.pages.dev/paper/W2579533542) · 7,742 citations
12. [NCBI prokaryotic genome annotation pipeline](https://nerdi.pages.dev/paper/W2470052926) · 7,052 citations
13. [The STRING database in 2017: quality-controlled protein–protein association networks, made broadly accessible](https://nerdi.pages.dev/paper/W2537623931) · 7,024 citations · Free PDF
14. [W-IQ-TREE: a fast online phylogenetic tool for maximum likelihood analysis](https://nerdi.pages.dev/paper/W2336400828) · 5,539 citations
15. [The GeneCards Suite: From Gene Data Mining to Disease Genome Sequence Analyses](https://nerdi.pages.dev/paper/W2474747771) · 5,503 citations
16. [Revised Estimates for the Number of Human and Bacteria Cells in the Body](https://nerdi.pages.dev/paper/W2952564192) · 5,423 citations · Free PDF
17. [PartitionFinder 2: New Methods for Selecting Partitioned Models of Evolution for Molecular and Morphological Phylogenetic Analyses](https://nerdi.pages.dev/paper/W2563535234) · 5,282 citations
18. [Interactive tree of life (iTOL) v3: an online tool for the display and annotation of phylogenetic and other trees](https://nerdi.pages.dev/paper/W2336330109) · 5,044 citations
19. [Dissecting the multicellular ecosystem of metastatic melanoma by single-cell RNA-seq](https://nerdi.pages.dev/paper/W2332292689) · 4,949 citations
20. [ggtree : an r package for visualization and annotation of phylogenetic trees with their covariates and other associated data](https://nerdi.pages.dev/paper/W2513727910) · 4,888 citations
21. [Sharing and community curation of mass spectrometry data with Global Natural Products Social Molecular Networking](https://nerdi.pages.dev/paper/W2504691963) · 4,611 citations · Free PDF
22. [UniProt: the universal protein knowledgebase](https://nerdi.pages.dev/paper/W4210702584) · 4,463 citations
23. [Ki67 expression in breast cancer](https://nerdi.pages.dev/paper/W2259802687) · 4,384 citations
24. [Comparative Protein Structure Modeling Using MODELLER](https://nerdi.pages.dev/paper/W4211196614) · 4,350 citations
25. [Dietary Advanced Glycation End Products and Their Potential Role in Cardiometabolic Disease in Children](https://nerdi.pages.dev/paper/W2302447233) · 4,011 citations

Canonical page: https://nerdi.pages.dev/papers/genetics-and-biochemistry/most-cited-2016