“Improved metagenomic analysis with Kraken 2” (2019), by Derrick E. Wood et al. Genetics and Biochemistry research paper. Original abstract below, unchanged. A free PDF is available.

Improved metagenomic analysis with Kraken 2

Derrick E. Wood, Jennifer Lu, Ben Langmead · November 28, 2019 · Genetics and Biochemistry

Abstract

Although Kraken's k-mer-based approach provides a fast taxonomic classification of metagenomic sequence data, its large memory requirements can be limiting for some applications. Kraken 2 improves upon Kraken 1 by reducing memory usage by 85%, allowing greater amounts of reference genomic data to be used, while maintaining high accuracy and increasing speed fivefold. Kraken 2 also introduces a translated search mode, providing increased sensitivity in viral metagenomics analysis.

Free PDF DOI: 10.1186/s13059-019-1891-0