“A subcellular map of the human proteome” (2017), by Peter J. Thul et al. Chemistry research paper. Original abstract below, unchanged. No free PDF was found.

A subcellular map of the human proteome

Peter J. Thul, Lovisa Åkesson, Mikaela Wiking et al. · May 11, 2017 · Chemistry

Abstract

Resolving the spatial distribution of the human proteome at a subcellular level can greatly increase our understanding of human biology and disease. Here we present a comprehensive image-based map of subcellular protein distribution, the Cell Atlas, built by integrating transcriptomics and antibody-based immunofluorescence microscopy with validation by mass spectrometry. Mapping the in situ localization of 12,003 human proteins at a single-cell level to 30 subcellular structures enabled the definition of the proteomes of 13 major organelles. Exploration of the proteomes revealed single-cell variations in abundance or spatial distribution and localization of about half of the proteins to multiple compartments. This subcellular map can be used to refine existing protein-protein interaction networks and provides an important resource to deconvolute the highly complex architecture of the human cell.

Source page DOI: 10.1126/science.aal3321